Polyene macrolide antibiotic nanoemulsion: a proposal for the treatment of cutaneous leishmaniasis

Leishmaniasis is a neglected tropical disease that requires timely and inexpensive treatment. For this purpose, nanoemulsion with polyene macrolide antibiotic, or amphotericin B (NE-AmB), was developed. This study quantified the amount of drug permeated retained in intact lacerated human skin, simulating cutaneous leishmaniasis (CL) processes. Toxicity macrophage keratinocyte cell lines, activity against promastigotes amastigotes Leishmania tropica, vivo irritant activity, histological evidence evaluated. Results. The damaged skin 750.18 ± 5.43 567.97 8.64 µg/g/cm2, respectively. There no permeation. No apparent toxic effect observed HaCaT lines. IC50 NE-AmB found for 0.26 0.09 0.37 0.05 µg/mL, NE without AmB did show antiparasitic activity. formulation showed lower values on both parasite stages than solution. irritation, histology improvement We suggest may be candidate studies CL patients. Keywords. Leishmaniasis, Amphotericin B, ex permeation studies, vitro cytotoxicity, leishmanicidal Draize test, histology.